Antiproliferative activity of biomass extract from Pseudomonas cedrina

Background: The study of plant-associated microorganisms is very important in the discovery and development of bioactive compounds. Pseudomonas is a diverse genus of Gammaproteobacteria comprising more than 60 species capable of establishing themselves in many habitats, which include leaves and stems of many plants. There are reports of metabolites with diverse biological activity obtained from bacteria of this genus, and some of the metabolites have shown cytotoxic activity against cancer cell lines. Because of the high incidence of cancer, research in recent years has focused on obtaining new sources of active compounds that exhibit interesting pharmacodynamic and pharmacokinetic properties that lead to the development of new therapeutic agents. Results: A bacterial strain was isolated from tumors located in the stem of Pinus patula, and it was identified as Pseudomonas cedrina. Extracts from biomass and broth of P. cedrina were obtained with chloroform:methanol (1:1). Only biomass extracts exhibited antiproliferative activity against human tumor cell lines of cervix (HeLa), lung (A-549), and breast (HBL-100). In addition, a biomass extract from P. cedrina was fractioned by silica gel column chromatography and two diketopiperazines were isolated: cyclo-(L-Prolyl-L-Valine) and cyclo-(L-Leucyl-L-Proline). Conclusions: This is the first report on the association of P. cedrina with the stems of P. patula in Mexico and the antiproliferative activity of extracts from this species of bacteria against human solid tumor cell lines.

Electrocoagulación monopolar de la arteria cística en colecistectomía laparoscópica: experiencia en el Hospital Central de Maracay / Monopolar electrocoagulation of cystic artery during laparoscopic cholecystectomy

Exponer la experiencia del uso de electrocoagulación monopolar de la arteria cística en la colescistectomia laparoscopia en el Servicio de Cirugía General Hospital Central de Maracay. Se realizó un estudio cuantitativo, prospectivo, descriptivo, transversal, basado en electrocoagulación monopolar de la arteria cìstica en colecistectomia laparoscópica electiva a 64 pacientes con arteria cística menor o igual a 2 mm, en un período de tiempo comprendido entre enero 2010 y abril 2011. Se evaluaron variables: edad, sexo, estadio clínico, presencia de sangrado de arteria cistica, presencia de lesiones de vías biliares, tiempo quirúrgico, conversión a colecistectomía abierta, estancia hospitalaria. 86% correspondio al sexo femenino. La edad promedio fue 39 años (rango de 13 a 73 años), siendo la patología litiasis vesicular, con tiempo quirúrgico de 46 a 60 minutos (rango 25 a 95 minutos), sin casos de sangrado de arteria cística, lesión de vía biliar ni conversión a cirugía abierta. Morbilidad en 27% de los casos, conformada por dolor en heridas operatorias, abdominal y nauseas, con estancia hospitaria de 24 horas en 97%, de 48 horas en 3%. La electrocoagulación monopolar de la arteria cística es un método seguro, efectivo que brinda una alternativa diferente en manejo de la misma.

To describe the experience of using monopolar electrocoagulation of the cystic artery in laparoscopy cholecystectomy in partients attending the outpatient minimally invasive laparoscopic surgery at the Hospital Central de Maracay. It was performed a quantitative prospective, cross sectional study, based on monopolar electrocoagulation of the cystic artery during laparoscopic cholecystectomy of 64 elective patients whose cystic artery was less than or equal to 2 mm over a period of time between January 2010 and april 2011. Variables were evaluated: age, sex, clinical stage, presence of cystic artery bleeding, presence of the bile duct injuries durat0ion of surgery, conversión to open cholecystectomy, hospital stay. 86% were females. The average age was 39 years (range 13 to 73 years), with gallstone disease with a surgical time of 46 to 60 minutes (range 25 to 95 minutes), no cases of bleeding from the cystic artery, injury bile duct or conversion to open surgery were reported, morbidity comprised of abdominal pain at the surgical wounds, nausea in 27% of the patients and hospital stay was 24 hours at 97% 48 hours on 3%. The monopolar electrocoagulation of the cystic artery is a sale, effective supplement that provides an alternative in the management of it.